mouse monoclonal st2 antibody  (Proteintech)

Journal: Scientific reports

Article Title: A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis.

doi: 10.1038/s41598-017-10465-0

Figure Lengend Snippet: Figure 1. Corticosteroid treatment increases serum levels of ST2 in UC. Serum ST2 levels in UC patients grouped according to therapies (A) or according to disease score activity and corticosteroid (CORT) treatment (B) were determined by ELISA. 5-ASA (5-aminosalicylic acid), AZA (azathioprine), corticosteroids (hydrocortisone, prednisone and prednisolone), IFX (infliximab). Differences were assessed using the Kruskal- Wallis test and Dunn’s multiple comparison post-test. *p < 0.05; **p < 0.01; ***p < 0.001. sST2 (C), IL6 (D) in conditional media of biopsies from healthy controls (HC) or UC patients, stimulated or not with 100 nM Dexametasone (Dex) for 24 hours. Differences between medians were assessed using the Mann-Whitney U-test. *p < 0.05; **p < 0.01.

Article Snippet: A mouse monoclonal antibody against human ST2 (MAB523, R&D Systems) and an Alexa 546-tagged secondary goat antibody against mouse IgG (Invitrogen/Life Technologies, Carlsbad, CA, USA) were used.

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Comparison, MANN-WHITNEY

Journal: Scientific reports

Article Title: A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis.

doi: 10.1038/s41598-017-10465-0

Figure Lengend Snippet: Figure 4. Corticosteroid treatment increases sST2 levels in UC patients with IL1RL1 genetic variants. Serum ST2 levels determined by ELISA in genotyped UC patients (A), controls and (B) UC patients receiving or not receiving corticosteroids (C). Kruskal-Wallis test with Dunn’s multiple comparison post-test. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: A mouse monoclonal antibody against human ST2 (MAB523, R&D Systems) and an Alexa 546-tagged secondary goat antibody against mouse IgG (Invitrogen/Life Technologies, Carlsbad, CA, USA) were used.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: TGFβ1 Suppresses IL-33-induced Mast Cell Function

doi: 10.4049/jimmunol.1601983

Figure Lengend Snippet: C57BL/6J BMMC were cultured for three days in IL-3 and SCF ±TGFβ1 (all at 10ng/ml). (A) Cells were stained with anti-T1/ST2 and analyzed by flow cytometry. (B and C) Cells were activated with IL-33 to detect intracellular cytokine production as described in Materials and Methods. Flow cytometry was used to gate on surface T1/ST2-hi BMMC, which were also stained intracellularly with anti-TNF or anti-IL-6. (B) shows represented gating of T1/ST2-hi cells. (C) shows geometric mean fluorescent intensity of TNF or IL-6 staining among T1/ST2-hi cells. Data shown are mean gMFI±SE from (A) triplicate samples of at least 2 separate experiments or (B and C) 18 samples from 2 independent experiments. *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001.

Article Snippet: Anti-mouse T1/ST2 monoclonal antibody (clone DJ8), FITC-conjugated or PE-conjugated rat IgG2b isotype control, PE-conjugated anti-mouse CD63, APC-conjugated anti-mouse CD107a, and PE-conjugated anti-mouse IgE were purchased from eBioscience (San Diego, CA).

Techniques: Cell Culture, Staining, Flow Cytometry

Journal: Cancer immunology research

Article Title: Stereotactic Radiotherapy Increases Functionally Suppressive Regulatory T Cells in the Tumor Microenvironment

doi: 10.1158/2326-6066.CIR-17-0040

Figure Lengend Snippet: The effect of IL33 on the post-RT increase of Tregs in tumors. A, The time course of the IL33 level in tumor lysates from different time points (1, 6, 24, 48, and 72 hours and 7 days post RT) measured by ELISA (n = 5 per group). B, Experimental design. C57BL/6 mice were injected subcutaneously with 5 × 105 B16/F10 cells on day 0. Mice received 10 Gy of RT on day 7. Mice received a mAb to ST2 (200 μg/ mouse, Anti-ST2 Ab) or vehicle intraperitoneally (i.p.) every 3 days, starting 1 day before RT. Tumors, DLNs, and spleens were harvested on day 14. C, Tumor growth curves of vehicle control- (dashed line) versus ST2 Ab-treated mice (solid line), with or without radiation (black or red, respectively). D, Representative flow plot of TIL-Tregs. E and F, Quantitative scatter plots of % Foxp3+ cells of tumor-infiltrating CD4+ cells (E), and the absolute number of TIL-Tregs per gram tumor weight (F) in ST2 Ab-treated versus vehicle-treated group (n = 5 per group, repeated ×2). Error bars, SEM; ***, P < 0.001; **, P < 0.01; *, P < 0.05, determined by two-way ANOVA (A, C), and an unpaired Student t test (E, F).

Article Snippet: Mouse anti-ST2/IL-33 R monoclonal antibody (Clone 245707, R&D) was diluted in PBS and administrated intraperitoneally at a concentration of 200 μg in a volume of 100 μL per mouse every 3 days ( 25 ), starting 1 day before RT, for a total of three doses.

Techniques: Enzyme-linked Immunosorbent Assay, Injection

Journal: Cancer immunology research

Article Title: Stereotactic Radiotherapy Increases Functionally Suppressive Regulatory T Cells in the Tumor Microenvironment

doi: 10.1158/2326-6066.CIR-17-0040

Figure Lengend Snippet: The effect of IL33 on the post-RT increase of Tregs in tumors. A, The time course of the IL33 level in tumor lysates from different time points (1, 6, 24, 48, and 72 hours and 7 days post RT) measured by ELISA (n = 5 per group). B, Experimental design. C57BL/6 mice were injected subcutaneously with 5 × 105 B16/F10 cells on day 0. Mice received 10 Gy of RT on day 7. Mice received a mAb to ST2 (200 μg/ mouse, Anti-ST2 Ab) or vehicle intraperitoneally (i.p.) every 3 days, starting 1 day before RT. Tumors, DLNs, and spleens were harvested on day 14. C, Tumor growth curves of vehicle control- (dashed line) versus ST2 Ab-treated mice (solid line), with or without radiation (black or red, respectively). D, Representative flow plot of TIL-Tregs. E and F, Quantitative scatter plots of % Foxp3+ cells of tumor-infiltrating CD4+ cells (E), and the absolute number of TIL-Tregs per gram tumor weight (F) in ST2 Ab-treated versus vehicle-treated group (n = 5 per group, repeated ×2). Error bars, SEM; ***, P < 0.001; **, P < 0.01; *, P < 0.05, determined by two-way ANOVA (A, C), and an unpaired Student t test (E, F).

Article Snippet: IL33 signaling blockade Mouse anti-ST2/IL-33 R monoclonal antibody (Clone 245707, R&D) was diluted in PBS and administrated intraperitoneally at a concentration of 200 μg in a volume of 100 μL per mouse every 3 days ( 25 ), starting 1 day before RT, for a total of three doses.

Techniques: Enzyme-linked Immunosorbent Assay, Injection

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Th2 related markers in milk allergic inflammatory mice model, versus OVA

doi: 10.1016/j.jgeb.2017.07.001

Figure Lengend Snippet: Intranasal challenge with low fat milk increases the numbers of T1/ST2 + cells in lung. (A) T1/ST2 + cells (FITC-green) was detected using immunofluorescence microscopy on consecutive frozen lung sections from OVA, low fat milk treated mice and naïve control mice (n = 6 per group). The blue color demonstrates the nuclear staining by DAPI dye. Magnification is 60×. (B) Immunofluorescence analysis for quantification of T1/ST2 + cells relative to the area of lung. We scored the percentage of T1/ST2 positive cells in the different groups compared to the DAPI positive cells in the same area. We calculated the number of the expressed T1/ST2 cells in 10 lung fields with 1000× magnification, in a total area of 15 × 10 4 µm 2 , based on dFOV (diameter of field of view).

Article Snippet: Thereafter, lung sections were stained with FITC-labeled T1/ST2 rat anti-mouse monoclonal antibody (R&D systems, Minneapolis, MN, USA) for 60 min at 37 °C, followed by 3 times wash with PBS for 3 min each.

Techniques: Immunofluorescence, Microscopy, Staining